ELECTRON MICROSCOPE RADIOAUTOGRAPHY AS A QUANTITATIVE TOOL IN ENZYME CYTOCHEMISTRY
نویسندگان
چکیده
منابع مشابه
Electron Microscope Radioautography as a Quantitative Tool in Enzyme Cytochemistry
Tritiated diisopropylfluorophosphate (DFP) was used to phosphorylate acetylcholinesterase (AChase) in the motor end plate of mouse sternomastoid muscle, and its distribution within the end plate was evaluated quantitatively by electron microscope radioautography. With the use of emulsion layers whose sensitivity to tritium had been calibrated, the density of AChase in different components of th...
متن کاملResolution in Electron Microscope Radioautography
Experimental resolution values, half distances (HD), were determined for electron microscope radioautography with (14)C as the source of radioactivity. These were about a factor of 1.5-2 times higher than for tritium. Grain distributions normalized in units of HD were found to fit the "universal" curves previously obtained for tritium.
متن کاملAbsolute Sensitivity of Electron Microscope Radioautography
A calibration method is described for measuring absolute radioautographic sensitivities under various experimental conditions. Sensitivities to (3)H and (35)S radiation, i.e. ratio of developed grains to radioactive decays in the specimen, were determined with Ilford L4 and Kodak NTE emulsions. The highest values obtained in monolayers of emulsion were (1/8) for (3)H and 1/21 for (35)S. The inf...
متن کاملQuantitative enzyme cytochemistry of leukaemic cells.
A new technique for the quantitative estimation of glycolytic and respiratory enzyme activity in intact and unfixed bone marrow and peripheral blood cells is described. The method gives a two- to three-fold increase in demonstrable enzyme activity per cell compared with existing techniques using fixed marrow film preparations, and is particularly applicable to the study of relatively fragile ce...
متن کاملRna Synthesis in Rat and Mouse Hepatic Cells as Studied with Light and Electron Microscope Radioautography
Work on radioautographic localization of RNA synthesis has been reviewed by Prescott (1). The findings with this method show good correspondence with biochemical evidence by indicating the nucleus as the presumptive site of RNA synthesis, and the probability of transport of RNA from the nucleus to the cytoplasm has been established. The method has been applied by Nossal et al. (2) in semiquanti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1969
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.42.1.122